2OV0
Structure of the blue copper protein Amicyanin to 0.75 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-03-07 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.65260 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.540, 55.890, 27.160 |
| Unit cell angles | 90.00, 95.61, 90.00 |
Refinement procedure
| Resolution | 20.000 - 0.750 |
| R-factor | 0.1251 |
| Rwork | 0.125 |
| R-free | 0.14180 |
| Structure solution method | AB INITIO |
| Starting model (for MR) | 1aac |
| RMSD bond length | 0.016 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 0.760 |
| High resolution limit [Å] | 0.750 | 2.040 | 0.750 |
| Rmerge | 0.050 | 0.021 | 0.351 |
| Number of reflections | 105738 | ||
| <I/σ(I)> | 9 | ||
| Completeness [%] | 97.8 | 99.4 | 93.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.5 | 293 | 3 M sodium phosphate, pH 5.5, EVAPORATION, temperature 293K |
