2OUM
The first domain of L1 from Thermus thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-19 |
Detector | MAR CCD 165 mm |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.700, 45.320, 37.890 |
Unit cell angles | 90.00, 100.74, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.550 |
R-factor | 0.18147 |
Rwork | 0.177 |
R-free | 0.26973 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | The first domain of L1 from Thermus thermophilus |
RMSD bond length | 0.010 |
RMSD bond angle | 1.320 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.600 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.160 | 0.431 |
Number of reflections | 3392 | |
<I/σ(I)> | 8.8 | 3.5 |
Completeness [%] | 94.4 | 89.1 |
Redundancy | 3.3 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 277 | 30% v/v polyethylenglycol 4K, 100 mM Tris-HCl, 200 mM acetate Na, pH 8.5, VAPOR DIFFUSION, temperature 277K |