2OUK
ABC Protein ArtP in complex with Sulphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-04-28 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.97973 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.164, 80.628, 95.584 |
| Unit cell angles | 90.00, 104.23, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.150 |
| Rwork | 0.211 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SWISS-MODEL GENERATED MODEL |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.251 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.087 | 0.465 |
| Number of reflections | 55290 | |
| <I/σ(I)> | 7 | 1.45 |
| Completeness [%] | 94.2 | 80.3 |
| Redundancy | 3.3 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 23% PEG 3350, 0.1M BisTris, 0,2M lithium sulphate, 1,5% CAPB (TEGO BetainF), pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
