2OUE
Crystal structure of a junctionless all-RNA hairpin ribozyme at 2.05 angstroms resolution
Replaces: 1ZFRExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-28 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9764 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 93.272, 93.272, 131.250 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.400 - 2.050 |
| R-factor | 0.252 |
| Rwork | 0.252 |
| R-free | 0.26800 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.400 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 21699 | |
| <I/σ(I)> | 24 | 3.3 |
| Completeness [%] | 99.3 | 99.1 |
| Redundancy | 8.2 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG 2000 MME, LITHIUM SULFATE, cacodylate, spermidine, cobalt hexaamine, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 2000 MME | ||
| 10 | 1 | 2 | cobalt hexaamine | ||
| 2 | 1 | 1 | LITHIUM SULFATE | ||
| 3 | 1 | 1 | cacodylate | ||
| 4 | 1 | 1 | spermidine | ||
| 5 | 1 | 1 | cobalt hexaamine | ||
| 6 | 1 | 2 | PEG 2000 MME | ||
| 7 | 1 | 2 | LITHIUM SULFATE | ||
| 8 | 1 | 2 | cacodylate | ||
| 9 | 1 | 2 | spermidine |
