2OUA
Crystal Structure of Nocardiopsis Protease (NAPase)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 61.605, 61.605, 184.479 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.180 - 1.850 |
| Rwork | 0.176 |
| R-free | 0.19990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | poly-Ala model of TFPA (to be submitted) |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.180 | |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.097 | 0.469 |
| Number of reflections | 34991 | |
| <I/σ(I)> | 16.66 | 3.91 |
| Completeness [%] | 97.4 | 98.7 |
| Redundancy | 5.4 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 1.3M ammonium sulfate, 10%(v/v) dioxane, 0.1M MES, pH 6.0, 10mg/ml NAPase, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
