2OU9
Structure of Spin-labeled T4 Lysozyme Mutant T115R1/R119A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-10-03 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 60.406, 60.406, 96.373 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 1.550 |
R-factor | 0.1989 |
Rwork | 0.159 |
R-free | 0.21970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lzm |
RMSD bond length | 0.010 |
RMSD bond angle | 0.027 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | SHELX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 32082 | |
<I/σ(I)> | 37.4 | 1.9 |
Completeness [%] | 96.5 | 76.4 |
Redundancy | 8.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.9 | 296 | 2.0 M Na/K Phosphate, 240 mM NaCl, 40 mM 2-hydroxyethyl disulfide, pH 6.9, VAPOR DIFFUSION, temperature 296K |