2OU9

Structure of Spin-labeled T4 Lysozyme Mutant T115R1/R119A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-10-03
Detector	RIGAKU RAXIS IV
Spacegroup name	P 32 2 1
Unit cell lengths	60.406, 60.406, 96.373
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.550
R-factor	0.1989
Rwork	0.159
R-free	0.21970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lzm
RMSD bond length	0.010
RMSD bond angle	0.027
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	SHELX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.000	1.550
High resolution limit [Å]	1.500	1.500
Number of reflections	32082
<I/σ(I)>	37.4	1.9
Completeness [%]	96.5	76.4
Redundancy	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.9	296	2.0 M Na/K Phosphate, 240 mM NaCl, 40 mM 2-hydroxyethyl disulfide, pH 6.9, VAPOR DIFFUSION, temperature 296K