2OTT
Crystal structure of CD5_DIII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2006-08-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 74.161, 74.161, 128.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.690 - 2.500 |
| R-factor | 0.21842 |
| Rwork | 0.216 |
| R-free | 0.25863 |
| Structure solution method | SAD |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.409 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.650 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.070 | 0.340 |
| Number of reflections | 12350 | |
| <I/σ(I)> | 6.9 | 2.1 |
| Completeness [%] | 99.6 | 98.9 |
| Redundancy | 8.9 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.4 | 289 | 10mM Hepes, 300mM Na,Cl pH7.4, VAPOR DIFFUSION, temperature 289K |
