2OTM
Crystal structure of a putative endoribonuclease (so_1960) from shewanella oneidensis mr-1 at 1.85 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-01-31 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91837, 0.97931, 0.97885 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 112.083, 62.908, 78.689 |
Unit cell angles | 90.00, 93.24, 90.00 |
Refinement procedure
Resolution | 29.210 - 1.850 |
R-factor | 0.152 |
Rwork | 0.151 |
R-free | 0.17700 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.484 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.210 | 29.210 | 1.900 |
High resolution limit [Å] | 1.850 | 8.270 | 1.850 |
Rmerge | 0.105 | 0.052 | 0.667 |
Total number of observations | 1958 | 13148 | |
Number of reflections | 46806 | ||
<I/σ(I)> | 5.9 | 10.8 | 1.1 |
Completeness [%] | 100.0 | 97.8 | 100 |
Redundancy | 3.8 | 3.6 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | NANODROP, 0.16M Ca(OAc)2, 20.0% Glycerol, 14.4% PEG 8000, 0.1M Cacodylate pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |