2OTG
Rigor-like structures of muscle myosins reveal key mechanical elements in the transduction pathways of this allosteric motor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.087, 51.689, 159.900 |
| Unit cell angles | 90.00, 100.77, 90.00 |
Refinement procedure
| Resolution | 19.970 - 3.120 |
| Rwork | 0.276 |
| R-free | 0.30400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sr6 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 22033 | |
| <I/σ(I)> | 11.8 | 1.98 |
| Completeness [%] | 87.3 | 38.2 |
| Redundancy | 3.3 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 15% PEG 2K, 5mM MgCl2, 100mM SrCl2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
