2OTF
Crystal structure of the complex formed between phospholipase A2 and atenolol at 1.95 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 292 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-01-11 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54132 |
| Spacegroup name | P 43 |
| Unit cell lengths | 53.280, 53.280, 48.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.840 - 1.950 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2arm |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.840 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 9526 | |
| Completeness [%] | 95.5 | 92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | Ammonium Sulphate, PEG 4000, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
