2OT9
Crystal structure of YaeQ protein from Pseudomonas syringae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97980 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.311, 61.405, 77.828 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.220 - 1.970 |
| R-factor | 0.18034 |
| Rwork | 0.178 |
| R-free | 0.23911 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.270 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.077 | 0.284 |
| Number of reflections | 14144 | |
| <I/σ(I)> | 43.8 | 6.4 |
| Completeness [%] | 99.9 | 99.2 |
| Redundancy | 8.9 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 298 | 0.5M K/Na Tartrate, 0.2M LiSO4, 10% PEG3350, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
