2OSZ

Structure of Nup58/45 suggests flexible nuclear pore diameter by intermolecular sliding

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-15
Detector	ADSC QUANTUM 315
Spacegroup name	P 43 2 2
Unit cell lengths	92.705, 92.705, 169.553
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.850
Rwork	0.250
R-free	0.28800
Structure solution method	SAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	2.950
High resolution limit [Å]	2.850	6.090	2.850
Rmerge	0.098	0.047	0.389
Number of reflections	17216
<I/σ(I)>	8.8
Completeness [%]	95.7	89	98.1
Redundancy	4.2	4.1	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.1	298	100 mM Bis-Tris, 600 mM CaCl2, 2.7% (w/v) Benzamidine-HCl, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K