2OSW
Endo-glycoceramidase II from Rhodococcus sp.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-11-11 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.750, 92.941, 94.545 |
| Unit cell angles | 90.00, 98.55, 90.00 |
Refinement procedure
| Resolution | 29.570 - 1.600 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.20400 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.059 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.979 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.053 | 0.335 |
| Total number of observations | 111092 | |
| Number of reflections | 119859 | |
| <I/σ(I)> | 9.5 | 2.2 |
| Completeness [%] | 99.2 | 98.2 |
| Redundancy | 6.4 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 20% (w/v) PEG 3350; 0.175 M NaCl; 0.1 M Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
