2OSE
Crystal Structure of the Mimivirus Cyclophilin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-30 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 95.032, 95.032, 95.032 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.270 - 2.040 |
| R-factor | 0.216 |
| Rwork | 0.214 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bit |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.489 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.270 | 2.110 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.067 | 0.712 |
| Number of reflections | 18528 | |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.8 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1 M sodium phosphate monobasic monohydrate, 0.1 M potassium phosphate monobasic, 0.1 M MES monohydrate (pH 6.5), and 2 M sodium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
