2OS7
Caf1M periplasmic chaperone tetramer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 141.092, 174.267, 60.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| R-factor | 0.25157 |
| Rwork | 0.249 |
| R-free | 0.29843 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Caf1M (chain A) from pdb entry 1p5v |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.654 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 200.000 | 3.160 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.165 | 0.400 |
| Number of reflections | 33968 | |
| <I/σ(I)> | 3.8 | 3.8 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 8% PEG 6000 in 50 mM Tris-HCl pH 8.0-8.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
