2OS2
Crystal structure of JMJD2A complexed with histone H3 peptide trimethylated at Lys36
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-01-18 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.54180 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 100.943, 148.886, 56.975 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.17863 |
| Rwork | 0.176 |
| R-free | 0.22552 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2oq7 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.059 | |
| Number of reflections | 38324 | |
| <I/σ(I)> | 20 | 1.9 |
| Completeness [%] | 98.4 | 93.3 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 20% PEG 3350, 0.1 M Citrate, 2 mM NiCl2, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
