2OS1
Structures of actinonin bound peptide deformylases from E. faecalis and S. pyogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6B |
| Synchrotron site | PAL/PLS |
| Beamline | 6B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-26 |
| Detector | BRUKER PROTEUM 300 |
| Wavelength(s) | 1.12714 |
| Spacegroup name | P 43 |
| Unit cell lengths | 67.999, 67.999, 41.127 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.040 - 1.500 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.19800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lqy |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.052 | 0.283 |
| Number of reflections | 37104 | |
| <I/σ(I)> | 28.3 | 4.5 |
| Completeness [%] | 86.4 | 19.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 294 | 16% PEG 8000, 0.2M Ammonium sulfate, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
