2ORZ

Structural Basis for Ligand Binding and Heparin Mediated Activation of Neuropilin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CAMD BEAMLINE GCPCC
Synchrotron site	CAMD
Beamline	GCPCC
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-13
Detector	MAR CCD 165 mm
Spacegroup name	P 21 21 21
Unit cell lengths	50.951, 64.653, 102.308
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.340 - 2.150
R-factor	0.20641
Rwork	0.204
R-free	0.25675
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2orx
RMSD bond length	0.008
RMSD bond angle	1.191
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.150
Number of reflections	18747

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	0.1 M magnesium chloride, 0.1 M sodium cacodylate, 15% PEG 4K, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K