2ORY
Crystal structure of M37 lipase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2005-07-06 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.694, 95.102, 99.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.200 |
Rwork | 0.204 |
R-free | 0.24000 |
Structure solution method | MIR |
RMSD bond length | 0.010 |
RMSD bond angle | 1.420 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 34410 | |
<I/σ(I)> | 11.7 | 3.6 |
Completeness [%] | 98.8 | 99 |
Redundancy | 6.3 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.2M Ammonium acetate, 23-25% PEG 3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |