2ORI
Crystal structure of a thermostable mutant of Bacillus subtilis Adenylate Kinase (A193V/Q199R/)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-11-05 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 34.694, 75.136, 77.523 |
Unit cell angles | 90.00, 98.37, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
Rwork | 0.228 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2eu8 |
Data scaling software | d*TREK |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.990 | 19.990 | 1.660 |
High resolution limit [Å] | 1.600 | 3.440 | 1.600 |
Rmerge | 0.052 | 0.034 | 0.242 |
Total number of observations | 17233 | 11853 | |
Number of reflections | 49183 | ||
<I/σ(I)> | 16.6 | 50.1 | 4.4 |
Completeness [%] | 94.8 | 99.4 | 84.9 |
Redundancy | 3.04 | 3.18 | 2.69 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | CHESS, PEG 1500, CaCl2, pH 9.0, vapor diffusion, hanging drop, temperature 293K |