2ORE
Binary Structure of Escherichia coli DNA Adenine Methyltransferase and S-adenosylhomocysteine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2005-04-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 160.135, 160.135, 94.004 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.670 - 2.990 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g1p |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | GLRF |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.670 | 3.100 |
| High resolution limit [Å] | 2.990 | 2.990 |
| Rmerge | 0.111 | |
| Number of reflections | 28125 | |
| Completeness [%] | 99.5 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 289 | 1.5 M lithium sulfate,50mM magnesium sulfate, 100mM HEPES pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
