2ORE
Binary Structure of Escherichia coli DNA Adenine Methyltransferase and S-adenosylhomocysteine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 173 |
Detector technology | CCD |
Collection date | 2005-04-18 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97923 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 160.135, 160.135, 94.004 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.670 - 2.990 |
R-factor | 0.202 |
Rwork | 0.202 |
R-free | 0.23500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g1p |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | GLRF |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.670 | 3.100 |
High resolution limit [Å] | 2.990 | 2.990 |
Rmerge | 0.111 | |
Number of reflections | 28125 | |
Completeness [%] | 99.5 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 289 | 1.5 M lithium sulfate,50mM magnesium sulfate, 100mM HEPES pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K |