2OR4
A high resolution crystal structure of human glutamate carboxypeptidase II in complex with quisqualic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-12 |
| Detector | MAR CCD 225 mm |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 102.007, 130.437, 159.508 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.620 |
| R-factor | 0.18593 |
| Rwork | 0.184 |
| R-free | 0.21908 |
| Structure solution method | Rigid body |
| Starting model (for MR) | 2oot |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.939 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.680 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.070 | 0.465 |
| Number of reflections | 129603 | |
| <I/σ(I)> | 28.2 | 2.7 |
| Completeness [%] | 96.7 | 74.8 |
| Redundancy | 11 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 33% (v/v) pentaerythritol propoxylate PO/OH 5/4, 13% (w/v) PEG 3350, 100 mM Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
