2OR1
RECOGNITION OF A DNA OPERATOR BY THE REPRESSOR OF PHAGE 434. A VIEW AT HIGH RESOLUTION
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 148.300, 65.000, 27.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 2.500 |
| R-factor | 0.179 |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 * |
| High resolution limit [Å] | 2.500 * |
| Rmerge | 0.123 * |
| Number of reflections | 6019 * |
| Completeness [%] | 60.7 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4 * | VAPOR DIFFUSION, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | PEG 3000 | ||
| 3 | 1 | 1 | NACL | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 1 | SPERMINE | ||
| 6 | 1 | 2 | WATER |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | DNA | 1 (mM) | ||
| 2 | 1 | protein | 2 (mM) | ||
| 5 | 1 | PEG3000 | 12-14 (%) |
