2OR0
Structural Genomics, the crystal structure of a putative hydroxylase from Rhodococcus sp. RHA1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-16 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97924 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 99.881, 99.881, 407.920 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.730 - 2.100 |
| R-factor | 0.18554 |
| Rwork | 0.184 |
| R-free | 0.21764 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.377 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.730 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.144 | 0.526 |
| Number of reflections | 72190 | |
| <I/σ(I)> | 41.12 | 4.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 25.1 | 16.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.2M Ammonium acetate, 0.1M Bis-Tris, 20% PEG3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
