2OQY
The crystal structure of muconate cycloisomerase from Oceanobacillus iheyensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97890 |
| Spacegroup name | P 1 |
| Unit cell lengths | 104.635, 104.640, 104.462 |
| Unit cell angles | 109.50, 109.45, 109.47 |
Refinement procedure
| Resolution | 24.670 - 2.000 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.22900 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.067 |
| Number of reflections | 220117 |
| Completeness [%] | 95.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 22% PEG 3350, 0.1M HEPES, 0.2M Sodium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
