2OQT
Structural Genomics, the crystal structure of a putative PTS IIA domain from Streptococcus pyogenes M1 GAS
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97924, 0.97938 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.224, 112.781, 60.401 |
Unit cell angles | 90.00, 99.19, 90.00 |
Refinement procedure
Resolution | 35.850 - 2.410 |
R-factor | 0.21024 |
Rwork | 0.208 |
R-free | 0.25445 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.927 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.850 | 2.460 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.099 | 0.542 |
Number of reflections | 23714 | |
<I/σ(I)> | 34.86 | 2.02 |
Completeness [%] | 90.2 | 52.8 |
Redundancy | 6.7 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | 20% PEG3350, 0.2M Li2SO4, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |