2OQQ
Crystal structure of HY5 leucine zipper homodimer from Arabidopsis thaliana
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 6B |
Synchrotron site | PAL/PLS |
Beamline | 6B |
Wavelength(s) | 1.12714, 0.97880, 0.97895, 0.97114 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 48.344, 24.583, 76.517 |
Unit cell angles | 90.00, 100.03, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
Rwork | 0.243 |
R-free | 0.29600 |
Structure solution method | SIRAS |
RMSD bond length | 0.007 |
RMSD bond angle | 1.242 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.095 | 0.392 |
Number of reflections | 6080 | |
<I/σ(I)> | 9.1 | |
Completeness [%] | 97.7 | 87.8 |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 296 | 0.1M Bis-Tris pH6.0 0.1M MgCl2 25% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 296K |