2OQM

Crystal structure of a dinb family member protein (sden_0562) from shewanella denitrificans at 1.83 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-01-18
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837, 0.97908, 0.97874
Spacegroup name	P 1 21 1
Unit cell lengths	62.437, 71.722, 93.489
Unit cell angles	90.00, 107.35, 90.00

Refinement procedure

Resolution	45.220 - 1.830
R-factor	0.159
Rwork	0.157
R-free	0.19600
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.492
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.220		1.900
High resolution limit [Å]	1.830	3.130	1.830
Rmerge	0.065	0.042	0.467
Number of reflections	68483
<I/σ(I)>	8.61	16.7	2.2
Completeness [%]	92.9	97.6	83.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	NANODROP, 0.271M Ammonium formate, 18.5% Polyethylene glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K