2OQK
Crystal structure of putative Cryptosporidium parvum translation initiation factor eIF-1A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97970 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 94.769, 94.769, 113.883 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.930 - 1.800 |
| R-factor | 0.20709 |
| Rwork | 0.205 |
| R-free | 0.24099 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.464 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.074 | 0.780 |
| Number of reflections | 18459 | |
| <I/σ(I)> | 12.3 | 2.11 |
| Completeness [%] | 100.0 | 99.6 |
| Redundancy | 10.6 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 297 | 2.5 M Ammonium sulfate, 0.1M Sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
