2OQG
ArsR-like Transcriptional Regulator from Rhodococcus sp. RHA1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-01 |
| Detector | SBC-3 |
| Wavelength(s) | 0.9794, 0.9792, 0.9717 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.872, 57.494, 101.103 |
| Unit cell angles | 90.00, 96.54, 90.00 |
Refinement procedure
| Resolution | 28.940 - 1.540 |
| R-factor | 0.191 |
| Rwork | 0.188 |
| R-free | 0.25600 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.524 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.940 | 1.600 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.086 | 0.359 |
| Number of reflections | 52770 | |
| <I/σ(I)> | 7.9 | 2.43 |
| Completeness [%] | 92.4 | 52.4 |
| Redundancy | 8.1 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 295 | 0.1M Sodium acetate pH 4.5, 20% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
