2OQF
Structure of a synthetic, non-natural analogue of RNase A: [N71K(Ade), D83A]RNase A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2006-08-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.578, 50.534, 62.863 |
| Unit cell angles | 85.26, 74.31, 82.70 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.23555 |
| Rwork | 0.229 |
| R-free | 0.29120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nui |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.214 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 19122 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
