2OQE
Crystal Structure of Hansenula polymorpha amine oxidase in complex with Xe to 1.6 Angstroms
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-30 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 103.403, 222.751, 103.651 |
Unit cell angles | 90.00, 95.85, 90.00 |
Refinement procedure
Resolution | 46.680 - 1.600 |
R-factor | 0.163 |
Rwork | 0.162 |
R-free | 0.18800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a2v |
RMSD bond length | 0.010 |
RMSD bond angle | 1.352 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.080 | 0.373 |
Number of reflections | 587360 | |
<I/σ(I)> | 8.4 | |
Completeness [%] | 96.3 | 72.9 |
Redundancy | 2.7 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 298 | 8% PEG 8000, 0.3M potassium phosphate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K, pH 6.00 |