2OQB
Crystal structure of the N-terminal domain of coactivator-associated methyltransferase 1 (CARM1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-29 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 46.127, 46.127, 201.325 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.920 - 1.690 |
| R-factor | 0.209 |
| Rwork | 0.205 |
| R-free | 0.25500 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.242 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.920 | 1.750 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.052 | 0.106 |
| Number of reflections | 25232 | |
| <I/σ(I)> | 9.7 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.2 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 13%-16% PEG 6000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
