2OQA
X-ray Sequence and Crystal Structure of Luffaculin 1, a Novel Type 1 Ribosome-inactivating Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-05-22 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 1.05 |
Spacegroup name | P 1 |
Unit cell lengths | 39.135, 46.813, 83.571 |
Unit cell angles | 89.07, 80.01, 72.14 |
Refinement procedure
Resolution | 33.900 - 1.400 |
R-factor | 0.21313 |
Rwork | 0.213 |
R-free | 0.23157 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | a homology model built based on the sequence luffin a |
RMSD bond length | 0.007 |
RMSD bond angle | 1.153 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.900 | 1.436 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.030 | 0.030 |
Number of reflections | 94795 | |
<I/σ(I)> | 21.8 | 4.6 |
Completeness [%] | 86.7 | 62 |
Redundancy | 1.9 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 28% (w/v) PEG 6000, 0.1M citrate buffer(containing 0.02% (w/v) sodium azide)', pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |