2OQ7

The crystal structure of JMJD2A complexed with Ni and N-oxalylglycine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2006-09-23
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.99900
Spacegroup name	P 21 21 2
Unit cell lengths	101.120, 148.531, 57.333
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.150
R-factor	0.17876
Rwork	0.177
R-free	0.22800
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2gp3
RMSD bond length	0.017
RMSD bond angle	1.583
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.870	2.270
High resolution limit [Å]	2.150	2.150
Rmerge	0.078	0.485
Number of reflections	45023
<I/σ(I)>	10.4	2.1
Completeness [%]	94.5	96.5
Redundancy	3.2	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	20% PEG 3350, 0.1M citrate, 2 mM NiCl2, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K