2OQ0
Crystal Structure of the First HIN-200 Domain of Interferon-Inducible Protein 16
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-09 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.335, 88.959, 112.831 |
Unit cell angles | 90.00, 99.37, 90.00 |
Refinement procedure
Resolution | 41.310 - 2.000 |
R-factor | 0.211 |
Rwork | 0.208 |
R-free | 0.26000 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.332 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (refmac_5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.072 | 0.389 |
Number of reflections | 55105 | |
<I/σ(I)> | 14.3 | 2.7 |
Completeness [%] | 96.4 | 85.7 |
Redundancy | 4.1 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 26% PEG 6000, 0.1M TRIS-HCl pH 8.5, 0.2M MgCl2, VAPOR DIFFUSION, SITTING DROP, temperature 298K |