2OQ0
Crystal Structure of the First HIN-200 Domain of Interferon-Inducible Protein 16
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-09 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.335, 88.959, 112.831 |
| Unit cell angles | 90.00, 99.37, 90.00 |
Refinement procedure
| Resolution | 41.310 - 2.000 |
| R-factor | 0.211 |
| Rwork | 0.208 |
| R-free | 0.26000 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.332 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (refmac_5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.072 | 0.389 |
| Number of reflections | 55105 | |
| <I/σ(I)> | 14.3 | 2.7 |
| Completeness [%] | 96.4 | 85.7 |
| Redundancy | 4.1 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 26% PEG 6000, 0.1M TRIS-HCl pH 8.5, 0.2M MgCl2, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
