2OPW
Crystal structure of human phytanoyl-CoA dioxygenase PHYHD1 (apo)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.95520 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 91.970, 91.970, 81.610 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.060 - 1.900 |
| R-factor | 0.22108 |
| Rwork | 0.219 |
| R-free | 0.25793 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Model built from sulfur SAD data |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.569 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.060 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.120 | |
| Number of reflections | 31832 | |
| <I/σ(I)> | 10 | 2.24 |
| Completeness [%] | 99.0 | 92.5 |
| Redundancy | 7 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 7% PEG3350, 200mM Calcium acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
