2OPT
Crystal Structure of Apo ActR from Streptomyces coelicolor.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-08 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.648, 79.957, 104.027 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.370 - 2.050 |
| R-factor | 0.19767 |
| Rwork | 0.195 |
| R-free | 0.24655 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.594 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.370 | 2.030 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.055 | 0.543 |
| Number of reflections | 34133 | |
| <I/σ(I)> | 34.6 | 3.85 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.2 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 14% PEG4000, 91mM sodium acetate, 45mM Bis-Tris, 23mM KCl, 9.1mM EDTA, 4.6mM Tris, 0.45mM DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
