2OPS
Crystal Structure of Y188C Mutant HIV-1 Reverse Transcriptase in Complex with GW420867X.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-08-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.244 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 138.500, 110.300, 72.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.450 - 2.300 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.30100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.060 | 0.327 |
| Number of reflections | 49585 | |
| <I/σ(I)> | 17.3 | 2.4 |
| Completeness [%] | 98.6 | 96.5 |
| Redundancy | 3.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
