2OPJ
Crystal structure of O-succinylbenzoate synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-26 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.976, 37.642, 51.990 |
| Unit cell angles | 71.28, 76.13, 73.23 |
Refinement procedure
| Resolution | 34.940 - 1.600 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.22600 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.037 | 0.108 |
| Number of reflections | 29912 | |
| <I/σ(I)> | 11.3 | 12 |
| Completeness [%] | 90.5 | 52.7 |
| Redundancy | 3.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.1M Bis-Tris pH 5.5, 25% PEG3350, 0.2M Ammonium Acetate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
