2OPD
Structure of the Neisseria meningitidis minor Type IV pilin, PilX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 98 |
| Detector technology | CCD |
| Collection date | 2005-06-02 |
| Detector | BRUKER PROTEUM |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 66.128, 83.366, 105.323 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.500 |
| R-factor | 0.205 |
| Rwork | 0.204 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | P43 space group PilX |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.639 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 10281 | |
| <I/σ(I)> | 17.5 | 6.3 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 8.6 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | Protein at 7 mg/ml in 25 mM KCl, 50 mM Hepes, pH 7.5 was mixed in a 1:1 ratio with unbuffered 4 M sodium formate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
