2OPD
Structure of the Neisseria meningitidis minor Type IV pilin, PilX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 98 |
Detector technology | CCD |
Collection date | 2005-06-02 |
Detector | BRUKER PROTEUM |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 66.128, 83.366, 105.323 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.500 |
R-factor | 0.205 |
Rwork | 0.204 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | P43 space group PilX |
RMSD bond length | 0.016 |
RMSD bond angle | 1.639 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 10281 | |
<I/σ(I)> | 17.5 | 6.3 |
Completeness [%] | 99.8 | 100 |
Redundancy | 8.6 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | Protein at 7 mg/ml in 25 mM KCl, 50 mM Hepes, pH 7.5 was mixed in a 1:1 ratio with unbuffered 4 M sodium formate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |