2OPC

Structure of Melampsora lini avirulence protein, AvrL567-A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-04-22
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 21
Unit cell lengths	39.818, 52.379, 70.827
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.460 - 1.430
R-factor	0.19825
Rwork	0.197
R-free	0.22593
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.617
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.468
High resolution limit [Å]	1.430	1.432
Rmerge	0.056	0.238
Number of reflections	28149
<I/σ(I)>	33.7	13.95
Completeness [%]	93.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	290.05	10% PEG 8000, 0.1 M imidazole, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290.05K