2OOI
The crystal structure of gene product SA0254 from Staphylocococcus aureus subsp. aureus N315
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-02 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97940 |
Spacegroup name | P 63 |
Unit cell lengths | 116.104, 116.104, 86.185 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.170 - 2.600 |
R-factor | 0.22595 |
Rwork | 0.224 |
R-free | 0.27002 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.798 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.668 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.093 | 0.568 |
Number of reflections | 19380 | |
<I/σ(I)> | 28.57 | 2 |
Completeness [%] | 99.8 | 98.61 |
Redundancy | 14.5 | 10.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 298 | 2M Ammonium sulfate, 0.1M CAPS, 0.2M Lithium sulfate, pH 10.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |