2OOI
The crystal structure of gene product SA0254 from Staphylocococcus aureus subsp. aureus N315
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 63 |
| Unit cell lengths | 116.104, 116.104, 86.185 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.170 - 2.600 |
| R-factor | 0.22595 |
| Rwork | 0.224 |
| R-free | 0.27002 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.798 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.668 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.093 | 0.568 |
| Number of reflections | 19380 | |
| <I/σ(I)> | 28.57 | 2 |
| Completeness [%] | 99.8 | 98.61 |
| Redundancy | 14.5 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 298 | 2M Ammonium sulfate, 0.1M CAPS, 0.2M Lithium sulfate, pH 10.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
