2OOA
crystal structure of the UBA domain from Cbl-b ubiquitin ligase
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Detector technology | CCD |
Collection date | 2005-08-28 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 46.138, 50.199, 78.343 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 17.290 - 1.560 |
R-factor | 0.206 |
Rwork | 0.204 |
R-free | 0.23992 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.260 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.620 |
High resolution limit [Å] | 1.560 | 1.560 |
Rmerge | 0.071 | 0.215 |
Number of reflections | 13003 | |
<I/σ(I)> | 29.2 | 7.4 |
Completeness [%] | 97.5 | 89.5 |
Redundancy | 5.9 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 293 | 1.2M sodium/potassium phosphate, 12% glycerol, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |