2OO9
crystal structure of the UBA domain from human c-Cbl ubiquitin ligase
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F2 |
Synchrotron site | CHESS |
Beamline | F2 |
Detector technology | CCD |
Collection date | 2006-02-13 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 82.025, 82.025, 56.194 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.600 - 2.100 |
R-factor | 0.21838 |
Rwork | 0.216 |
R-free | 0.26009 |
Structure solution method | SAD |
RMSD bond length | 0.023 |
RMSD bond angle | 1.629 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.061 | 0.313 |
Number of reflections | 11572 | |
<I/σ(I)> | 18.2 | 4.9 |
Completeness [%] | 99.1 | 95.9 |
Redundancy | 7.3 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 3.8M sodium formate, 0.1M Tris, 4% (v/v) glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |