2OO2
Crystal structure of protein AF1782 from Archaeoglobus fulgidus, Pfam DUF357
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 44.631, 46.452, 43.177 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.242 |
| Rwork | 0.240 |
| R-free | 0.28900 |
| Structure solution method | SAD |
| RMSD bond length | 0.029 |
| RMSD bond angle | 1.954 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.193 | 1.890 |
| High resolution limit [Å] | 1.795 | 1.795 |
| Rmerge | 0.080 | 0.549 |
| Total number of observations | 7358 | |
| Number of reflections | 8690 | |
| <I/σ(I)> | 20.6 | 2.4 |
| Completeness [%] | 98.5 | 91.8 |
| Redundancy | 8.9 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 294 | 100mM Tris-HCl pH 7.5, 3.2M 1,6-hexanediol, 200mM Magnesium chloride, VAPOR DIFFUSION, temperature 294K |
