2ONO
Arg475Gln Mutant of Mitochondrial Aldehyde Dehydrogenase, apo form, pseudo-merohedrally twinned
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-03 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 101.472, 175.850, 101.458 |
| Unit cell angles | 90.00, 94.79, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.150 |
| R-factor | 0.251 |
| R-free | 0.31400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o05 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.013 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.061 | 0.198 |
| Number of reflections | 186854 | |
| <I/σ(I)> | 13.6 | |
| Completeness [%] | 97.5 | 87.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.6 | 293 | 100 mM ACES (N-[2-Acetamido]-2-aminoethane sufonic acid), 5 mM MgCl2, 100 mM Guanidine HCl, 18% w/v PEG 6000, 6 mM DTT, pH 6.6, vapor diffusion, temperature 293K, pH 6.60 |
