2ON9
Structure of an amyloid forming peptide VQIVYK from the repeat region of Tau
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-16 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 4.863, 61.926, 15.413 |
| Unit cell angles | 90.00, 98.11, 90.00 |
Refinement procedure
| Resolution | 30.960 - 1.510 |
| R-factor | 0.207 |
| Rwork | 0.206 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Extended beta strand VQIVYK |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.197 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 1.620 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.161 | 0.489 |
| Number of reflections | 1222 | |
| <I/σ(I)> | 3.8 | |
| Completeness [%] | 85.4 | 77 |
| Redundancy | 2.89 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | Peptide concentration: 30mg/ml, peptide:Reservoir ratio 1:1, Reservoir: 0.2M Ammonium acetate, 0.1M HEPES-Na, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
