2OMT
Crystal structure of InlA G194S+S/hEC1 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.81 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.240, 88.470, 110.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.000 |
R-factor | 0.199 |
Rwork | 0.194 |
R-free | 0.28300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1o6s |
RMSD bond length | 0.023 |
RMSD bond angle | 2.129 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | EPMR (2.5) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.121 | 0.370 |
Number of reflections | 36174 | |
<I/σ(I)> | 9.44 | 3.9 |
Completeness [%] | 95.8 | 91.5 |
Redundancy | 5 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | PEG 4000, CaCl2, Na-Acetate, Tris/MES, pH 6.0, vapor diffusion, hanging drop, temperature 298K |