2OMN
Bence Jones KWR Protein- Immunoglobulin Light Chain Dimer, P4(3)2(1)2 Crystal Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-11 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 68.630, 68.630, 181.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 - 2.200 |
| R-factor | 0.232 |
| Rwork | 0.226 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dcl |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.440 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.880 | 2.160 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Number of reflections | 20595 | |
| <I/σ(I)> | 23.1 | 2.5 |
| Completeness [%] | 76.8 | 15.1 |
| Redundancy | 9.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 1.5M Ammonium Sulfate, 0.1M Tris/HCl pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
